Top Tech Startups in Chicago, IL in 2025

Biotechnology is rewriting life as we know it, from the medicines we take, to the crops we grow, and the household goods that we rely on every day. But moving at the new speed of science requires better technology. Benchling’s mission is to unlock the power of biotechnology. The world’s most innovative biotech companies use Benchling’s R&D Cloud to power the development of breakthrough products.

The software used to manage networks hasn’t kept up with the dramatic expansion of networks themselves. The Forward Networks solution is designed to help network teams eliminate network outages and reduce time to remediate errors. Its platform enables engineers and operators to visualize and search complex networks, debug configuration problems, verify network-wide policy implementations, and predict network behavior prior to deploying changes. Forward Networks builds a software replica of an entire network to analyze its behavior. Using the network copy, the software traces, indexes, and stores the collected data, monitoring things like end-to-end behavior, verification of the network’s forwarding behavior and security posture, and close monitoring of configuration changes.

Pendo helps people adopt software more quickly and successfully. Pendo customers include the world’s leading software companies and digital enterprises, including Verizon, RE/MAX, LabCorp, OpenTable, Okta, Salesforce, and Zendesk. Founded in 2013 in the heart of Raleigh, North Carolina, Pendo is backed by Battery Ventures, Spark Capital, Meritech Capital and Sapphire Ventures. Through its ProductCraft and customer communities, sponsored events and podcast, Pendo aims to support the success of product and digital leaders everywhere. For more information, visit: pendo.io

Gusto is a modern, online people platform that helps small businesses take care of their teams. On top of full-service payroll, Gusto offers health insurance, 401(k)s, expert HR, and team management tools. Today, Gusto offices in Denver, San Francisco, and New York serve more than 100,000 businesses nationwide. Our customers come from all walks of life and so do we. We hire great people from a wide variety of backgrounds, not just because it's the right thing to do, but because it makes our company stronger. If you share our values and our enthusiasm for small businesses, you will find a home at Gusto.

Mashape powers API Driven Software with many leading products including Kong (Open-Source API Gateway), Galileo (API Analytics), Marketplace (API Hub) and Gelato (Dev Portals). Mashape is funded by tier 1 VC firms including Index Ventures, NEA, CRV, Stanford University, Jeff Bezos (Amazon) and Eric Schmidt (Google).

Klaviyo (pronounced “clay-vee-oh”) is a leading customer data and marketing automation platform dedicated to accelerating revenue and customer connection for online businesses. Klaviyo makes it easy to store, access, analyze and use transactional and behavioral data to power highly-targeted customer and prospect communications. The company's hybrid customer-data and marketing-platform model allows companies to grow by fostering direct relationships with customers, without giving up their valuable data to popular big-tech ad platforms. Over 275,000 innovative companies like Unilever, Custom Ink, Living Proof and Huckberry sell more with Klaviyo. Learn more at klaviyo.com. Learn more at klaviyo.com.

We help nurses (CNAs, LVNs, RNs) find work that fits their schedule close to home. There are 4 million nurses (second most popular job in the US). There are tens of thousands of healthcare facilities (such as hospitals, nursing homes, rehab centers) who need nurses. We bring them together by working with our healthcare facility clients to find the nurses they need.

Dataiku is the platform for Everyday AI, systemizing the use of data for exceptional business results. Organizations that use Dataiku elevate their people (whether technical and working in code or on the business side and low- or no-code) to extraordinary, arming them with the ability to make better day-to-day decisions with data. More than 500 companies worldwide use Dataiku to systemize their use of data, analytics, and AI, driving diverse use cases from fraud detection to customer churn prevention, predictive maintenance to supply chain optimization, and everything in between. Don’t get us wrong: we are a tech company building software. Our culture is even pretty geeky! But our driving force is and will always remain people, starting with ours. We consider our employees to be our most precious asset, and we are committed to ensuring that each of them gets the most rewarding, enjoyable, and memorable work experience with us. Headquartered in New York City, Dataiku was founded in Paris in 2013 and achieved unicorn status in 2019. Now, more than 800 employees work across the globe in our offices and remotely. Backed by a renowned set of investors and partners including CapitalG, Tiger Global, and Serena, we’ve set out to build the future of AI. Let’s do it together!

Our company is used by the largest, fastest-growing companies in the world. Powered by AI technology and leveraging advances in Natural Language Processing and Machine Learning in particular, our company is a revolutionary search engine that delivers superior results—helping agile teams make better strategic decisions and gain a competitive edge.

Today, on your smartphone you have an app store where you can find a dozen apps to have food delivered to your door, but we don’t have an app store where a large enterprise, like a bank, can find an app for income verification, or where an insurance company can find an app for processing claims. Every such application is custom built. We should wonder — why? The desktop computer is an important interface for business applications. Problem is, it wasn’t built to perform modern business operations. The problem lies in the operating system. Where Android and iOS were designed to allow applications to interact with one another, desktop computing simply wasn’t. This means that operations such as deal analysis and loan processing, which toggle between human data and computer data, can’t be performed without the assistance of a human being. It’s not only inefficient for businesses but utterly uninspiring for workers. Instabase is on a mission to fix that: to rethink the way we use apps and the way those apps work together to improve business processes and empower innovators to solve problems on a larger scale. Read More: about.instabase.com/our-story