Top Tech Startups in Ontario, Canada in 2026

Citylitics delivers predictive intelligence on local utility & public infrastructure markets The U.S. needs to invest $4.5 Trillion over the next 5 years to upgrade its infrastructure, including water, energy, public transit, roads, airports, and more. Citylitics is the only source of predictive intelligence on where, how, and why these infrastructure investments will be made. Our Data Engine transforms over a billion documents buried in 31,000+ cities & utilities data sources into high value intelligence for targeted business development. The largest infrastructure industry leaders rely on Citylitics for external datasets and intelligence reports Citylitics' vision is a world where data drives Trillions of dollars of societal infrastructure investment towards the most ideal social, economic, and environmental outcomes.

Iterative Health is pioneering the use of artificial intelligence-based precision medicine in gastroenterology (GI), with the aim of helping to optimize clinical trials investigating treatment of inflammatory bowel disease (IBD). We use advanced machine learning and computer vision to interpret endoscopic images along with other types of data, helping clinicians to better assess patients with potential GI problems. This gives practitioners enhanced capabilities in the detection, annotation, disease scoring and treatment of GI related diseases. Iterative Health is initially applying these advances towards standardizing disease severity characterization for inflammatory bowel disease. Ultimately, the company aims to establish more meaningful endpoints to serve as better predictors of both therapeutic response and disease outcomes. The company is headquartered in Cambridge, Massachusetts.

PurposeMed was founded in 2019 with the mission of improving access to complex care for underserved communities. We’ve grown quickly in our first few years, and now our exceptional team serves thousands of patients each month across our multiple verticals. PurposeMed consists of three complex care business units: Freddie, Frida, and Foria, and partners with Affirming Care Pharmacy. Freddie is focused on improving sexual health through education, prevention, and treatment with a focus on the LGBTQ2S+ community—we’re the #1 rated PrEP provider in North America, serving patients in both Canada and the USA! Frida is changing the lives of patients with easier access to adult ADHD diagnosis, and fast and affordable treatment. Foria Clinic provides trans, non-binary, and gender-diverse Canadians with gender-affirming healthcare. Affirming Care Pharmacy is a specialty pharmacy with locations in Calgary, Alberta, and Mississauga, Ontario. This partnership provides our patients with an end-to-end healthcare experience, from treatment, to prescription delivery, to continuous affirming care. We’re proud to be ranked #2 on Deloitte’s 2024 list of Canada’s 50 fastest-growing tech companies and recognized as one of Canada’s Most Admired Corporate Cultures for two consecutive years. Driven by a strong mission, our team is passionate about making healthcare accessible to those who need it most—and we make sure to have fun along the way!

Greenspace (greenspacehealth.com) is transforming mental health systems by improving the way that care is accessed, measured and delivered. Greenspace is a growth stage technology startup with offices in Toronto and Kelowna. Since launching in 2016, the platform has been deployed across thousands of mental health practices and organizations and expanding across Canada and the US. Mental illness has passed HIV/Aids, malaria and diabetes as the leading cause of disability in the world. While there is no cure, psychotherapy is the most cost and clinically effective form of treatment. Greenspace has developed solutions that draw on technology and clinical research to improve patient outcomes within this traditional psychotherapy model. Our Measurement Platform enables mental health providers and organizations to implement consistent evidence-based measurement into their practice. Our Intake Module facilitates a more effective referral into mental health and addictions services. Our Self-Guided Module is a population health solution that provides mental health support directly to individuals in various settings. We recently helped develop and launch Wellness Together Canada (ca.portal.gs) – a major initiative funded and launched by Health Canada to provide virtual mental health and substance use support for all Canadians through COVID-19.

Kritik is an early-stage but fast growing ed-tech startup working passionately to upgrade college classroom experience with our fun and automated peer-to-peer learning technology. We launched our product into +200 universities and are ready to scale in the upcoming year. We are serial entrepreneurs, backed by some of the top players in the Canadian startup ecosystem including ApplyBoard and GarageCapital. We are looking for smart and motivated people to join the startup and be part of the fast movement in changing the way students are tasked and assessed. Recently, we launched VisibleAI (a completely new product), for which we have developed Artificial Intelligence (AI) for educators and institutions and a platform for academic integrity to equip educators with practical, ready-to-use strategies and prompts while maintaining academic integrity and fostering learning. The goal is to ensure educators are well-equipped to leverage AI, the effectiveness of PBL, and related concepts. The product offers transparency by detecting and reporting AI-generated content, helping educators maintain rigorous academic standards while embracing AI's educational potential.

Bizwise builds AI tools that help small businesses launch, sell, and operate. Customers use our automated site generator, visual editor, and commerce features to run day-to-day.

Feeld is a dating app for the curious; for those open to experiencing people and relationships in a new way. Our mission is to elevate the human experience of sexuality and relationships and create a world where everyone is more intimately connected to each other and themselves.

Machinio.com is one of the world’s largest platforms connecting buyers and sellers of inventory and related services across multiple industries. Founded in 2013 and acquired in 2018, Machinio now serves over 4,000 paying clients globally and continues to expand its reach and impact. Thousands of dealers and suppliers rely on the platform to market and sell their equipment, connecting them with millions of prospective buyers each year. Machinio is widely recognized for leveraging technology to simplify and modernize the buying and selling process. By introducing innovative tools and improving marketplace efficiency, the company has helped both buyers and sellers save significant time while facilitating high-value transactions worldwide. Machinio System is Machinio’s all-in-one software solution built specifically for dealers and service providers. Launched in 2018, it combines modern, fully customizable, SEO-optimized websites with a fully integrated CRM platform for dealers. This unified solution enables businesses to manage their entire workflow in one place — including inventory, lead management, quoting, invoicing, contact databases, email marketing campaigns, and more. With continuous feature development and a strong product roadmap, Machinio System empowers dealers and service providers to maintain a professional online presence, leverage intelligent sales and marketing tools, and consistently generate and nurture business opportunities. Machinio has been featured in leading media outlets including Forbes, Business Insider, Inc., EquipmentWorld, Agriculture.com, and others.

Occupier is making it radically easier for businesses to make real estate decisions with our deal and portfolio management software. For traditional office tenants and growing retailers, real estate is typically the second largest expense for a business next to payroll, yet the technology used to manage it is no more sophisticated than antiquated accounting software, spreadsheets and email. This leaves companies vulnerable to existential risk due to missed lease obligations, slow decision making and misinformed real estate strategy. Occupier is set to change all of this with intuitive, automated workflow, enabling companies to utilize data and make more informed decisions that align with key business initiatives.

Bespoke Metrics is a fast-growing technology startup. We are a data-driven company aimed at disrupting the construction, insurance and real estate industries. We believe that even the smallest data point can provide valuable insight and can introduce these industries to a world of opportunity. Bespoke Metrics strives to provide innovative solutions for industries looking to utilize data to its fullest. We want to define your data before it defines you.