Principal Research Associate, NGS

About UsDeep Genomics is at the forefront of using artificial intelligence to transform drug discovery. Our proprietary AI platform decodes the complexity of RNA biology to identify novel drug targets, mechanisms, and therapeutics inaccessible through traditional methods. With expertise spanning machine learning, bioinformatics, data science, engineering, and drug development, our multidisciplinary team in Toronto and Cambridge, MA is revolutionizing how new medicines are created.

About the RoleThe NGS and Molecular Technology team is seeking a Principal Research Associate to support the molecular characterization and development of AI driven targets. We are looking for a highly independent, enthusiastic, and self-motivated individual to work on various aspects of screening, molecular, NGS, and cell technologies. This individual will join a dynamic, collaborative, and rapidly growing team focused on advancing discovery and validation of targets.

Key Responsibilities:

• Independently lead and develop new projects, experiments, and scientific processes to support the characterization and development of new assays and modalities.
• Actively undertake technology development and assay optimization efforts to enhance research capabilities.
• Keep up with technical advances in cell and molecular biology, innovating on assays and research projects.
• Design, execute, and analyze experiments involving mammalian cell culture, molecular biology techniques, and gene therapy applications.
• Perform nucleic acids extraction, quantitation, gel electrophoresis, PCRs, qPCRs, and other molecular biology techniques.
• Develop and optimize NGS pipelines, including Illumina-based DNA and RNA library construction, QC using TapeStation and fragment analyzer, and running MiSeq/NextSeq.
• Independently execute protocols, troubleshoot assays, and conduct literature searches for technology selection and onboarding.
• Maintain meticulous records of experimental procedures and results, including electronic lab notebook maintenance.
• Collaborate effectively in a cross-functional, multi-disciplinary environment to achieve research goals.

Basic Qualifications:

• B.S. with 6+ years or M.S. with 3+ years of relevant experience in cell and molecular biology, biotechnology, or a related field.
• Extensive experience in mammalian cell culture, including primary and cancer cell lines (e.g., U2OS, HEK293).
• Expertise in a broad range of molecular biology techniques and assay optimization.
• Proficiency in nucleic acids extraction, quantitation, gel electrophoresis, PCRs, qPCRs, and related techniques.
• Experience with NGS pipelines, including Illumina-based DNA and RNA library construction, QC, and sequencing.
• Strong attention to detail in executing experiments and maintaining records.
• Ability to work independently and collaboratively in a team-oriented environment.

Preferred Qualifications:

• Experience with mouse/human primary hepatocytes, iPSCs, and 3D cell culture.
• Familiarity with long-read sequencing technologies.
• Experience with liquid handling and automated NGS pipelines.
• Understanding of genome engineering technologies and their applications.

What we offer:

• A highly competitive salary, a meaningful equity ownership stake in Deep Genomics, and exceptional opportunities for learning and growth.
• Company paid benefits.
• You’ll also be joining a bright, collegial, highly-motivated group working at the intersection of two of the most exciting areas of science and technology: AI and biotechnology.